Turkish scientists have produced an aortic vein with a three-dimensional bio-printer. The study is a first in the world
Sabanji University faculty member Bahattin Koç and his team produced a three-dimensional bio-printer using living cells. The study, the first in the world, will be one of the steps of artificial organ production.
At Sabancı University Nanotechnology application and Research Center, the world's first 3-D bio-printer and aorta vessel were produced using human living cells. Associate professor of the Production Systems Program of the Faculty of Engineering and Natural Sciences of the University. Dr. Bahattin Koç and his team are also working to produce bifurcated vessels.
About the 300 thousand pounds project supported by TUBITAK, Assoc. Dr. Aries listed the reasons why they started with the aorta as follows: “Since the aorta is the largest and only vessel in the body, it is not possible to treat it with a vein that will be removed from another part of the body. Synthetic veins are used in the treatment, but it is not like a person's own veins. The second reason is that if we are going to produce tissues and organs with a 3-D printer, vascular tissue must be created to feed these tissues and organs. In our study, the abdominal (abdominal) aorta vessel will be able to produce using the patient's own cells or stem cells, and the artificial vessel will be able to be transplanted into patients. The pharmaceutical industry can conduct its trials in tissues produced in this way.”
Einstein's cause of death
“As a result of our study, the abdominal aortic vein can be produced using the patient's own cells or stem cells, and the artificial Vein will be transplanted to such patients,” said Aries, who explained that the cause of Einstein's death was abdominal (abdominal) aortic aneurysm. My head 3D living cells in bio-ink, used as bio-ink purchased the fibroblasts (connective tissue cells),indicating that reproduction has been achieved, the coach, said: “the most important goal of the project 3D bio-printer with normal tissue or even organs using stem cells from the patient's own cells and produce an exact copy of. This will eliminate the problems of rejection by the body. It's much harder to produce soft textures with a 3-D printer. But we're thinking of working in soft tissue.”
1 production in 2 hours
An aorta can be obtained in 1.5-2 hours with a 3-D printer. Tell process:
An MRI scan of an aortic vein tissue sample removes the data.
According to the anatomy of the tissue to be produced by the developed algorithms, the way the cells print is calculated.
Identifying support structures for cells. The difference in this study is that the cells support each other in accordance with the 3-D anatomy of the tissue.
Commands to control the bio-printer are saved to the file.
By using these commands, you control where the bio-printer will print the cell and where the support structure will print.
The printer prints large vascular tissue similar to the aorta, suitable for its anatomical structure, in 3-D using living cells and biomaterials.
They produced an aorta from a 39-year-old cell
For the first time in the world and in Turkey, tissue structure was produced using living cells with 3D bio-printer within the scope of ‘three-dimensional (3D) tissue and Organ printing project’ (3D Tissueand Organ Printing).
At Sabancı University Nanotechnology application and Research Center (Faculty of Engineering and Natural Sciences Production Systems Program, Bahattin Koç and his team managed to produce artificial tissue using three-dimensional bio-printing method using living cells.
The ultimate goal of the 3D tissue and organ printing project group, led by Bahattin Koç, is to print a part of a tissue or organ in a laboratory environment using living cells with a three-dimensional biofuels in accordance with its anatomical structure.The most important goal of the project is to be able to produce an exact copy of the required tissue or organ using the patient's own normal cells or stem cells using a 3D bio-printer. Thus, the artificial tissue or organ produced by the patient's own cells can eliminate a condition such as rejection of the patient's body.
The team was created using MRI data, in accordance with the anatomical structure in MRI, for the first time in Turkey and in the world by printing a sample of aortic vessel tissue, cells and support structures in three dimensions fold-by-fold.For the first time, the project team produced large vascular tissue similar to the aorta, suitable for its anatomical structure, using 3D bio-printing method using living cells. Unlike other techniques on Earth, researchers at Sabancı University have made a first in the world by printing tissue with 3D structures that support their “own-cat”.
About the three-dimensional (3B) tissue and Organ printing project
The three-dimensional (3D) tissue and Organ printing project team used living human fibroblast cells as bio-ink to 3D print a sample of aortic tissue.Human vascular tissue generally consists of three different cell types: Fibroblast, endothelial, and smooth novelized cells. Fibroblast cells are the main cells of connective tissue. It provides the formation of extracellularmatric structure and collagen protein necessary for tissues. Endothelial cells, on the other hand, are the cells that make up the thin inner layer of veins.Smooth Nov NOV is a type of muscle cell that is found in the intestines and in the esophagus and intestines, where the muscle cells are found in the internal organs, such as the veins.As a next step, studies are continuing to strengthen the vascular tissue in the bioreactor, which will be created using endothelium and smooth muscle cells, as well as phibroblasts Nov.
As a result of an interdisciplinary study, Production Systems, Biology, nanotechnology, materials, graduate students and researchers working on issues such as that included in a four-man team, the project also receives support from the consultants on medical issues, Yeditepe University and in biology at GATA.
Sabancı University Faculty of Engineering and Natural Sciences Production Systems Program lecturer Bahattin Koç describes the 3D tissue and Organ printing project
There are two main reasons why we prioritize this study: first, since the aorta is the largest and only vessel in a person, it is unfortunately not possible to treat it with otologgreft. For the treatment of this, synthetic veins made of plastic (dacron) are currently used, and they never look like normal human veins. The second reason is that if we are going to produce 3D artificial tissue or organs, vascular tissue must first be created to feed these tissues or organs. By the way, a fact that many people do not know: Einstein's cause of death is an abdominal aortic aneurysm. An aneurysm means that a vein expands like a balloon. At a later stage, it can cause internal bleeding and death with a ruptured vein. As a result of our study, the abdominal aortic vein can be produced using the patient's own cells or stem cells, and the artificial Vein will be transplanted into such patients. We are currently in the initial stages of these studies and their clinical application could take many years.
Unlike previous tissue engineering studies, we use living cells as bio-ink in 3D printing, using the algorithms we have developed to optimally calculate the printing ways of cells according to the anatomy of the tissue we will produce. At the same time, we determine the support structures to support the cells. Again, our biggest difference from other studies is that we determine according to the three-dimensional anatomy of the tissue, so that all cells fully support each other. Commands to control biofuels are saved to the file. Then, using these commands, we control where the bio-printer prints the cell, where the support structure.
As a result, our main goal is to obtain artificial aortic vascular tissue that is close to the anatomical structure and can meet physiological requirements. In the project, we do not make a functional organ or tissue, but we design a part of the tissue or organ one-on-one with the algorithms and programs that we have developed. Then we convert 3D biofuels into commands to control them and print them with living cells and biomaterials. At the moment, we are not at the stage of producing a fully functional artificial tissue, but we are working on this.